Application
Western blot analysis of MGAT3 antibody (N-term) (Cat.# AP2411a) in mouse spleen tissue lysates (35ug/lane). MGAT3 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | MGAT3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGAT3; GGNT3; Beta-1, 4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase; N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GGNT3 |
|---|---|
| Alternative Names | MGAT3 GGNT3 Beta-1 4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III |
| Molecular Weight(MW) | 61313 Da |
| Function | It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides |
| Tissue Specificity | This MGAT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MGAT3. |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein |
Application
Western blot analysis of MGAT3 antibody (N-term) (Cat.# AP2411a) in mouse spleen tissue lysates (35ug/lane). MGAT3 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
