Application
MGAT5 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MGAT5 antibody detected the MGAT5 protein (arrow).Application
MGAT5 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MGAT5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGAT5; GGNT5; Alpha-1, 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A; Alpha-mannoside beta-1, 6-N-acetylglucosaminyltransferase; GlcNAc-T V; Mannoside acetylglucosaminyltransferase 5; N-acetylglucosaminyl-transferase V |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GGNT5 |
|---|---|
| Alternative Names | MGAT5 GGNT5 Alpha-1 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A Alpha-mannoside beta-1 6-N-acetylglucosaminyltransferase GlcNAc-T V Mannoside acetylglucosaminyltransferase 5 N-acetylglucosaminyl-transferase V |
| Molecular Weight(MW) | 84543 Da |
| Function | Catalyzes the addition of N-acetylglucosamine in beta 1- 6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides |
| Tissue Specificity | This MGAT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 651-681 amino acids from the C-terminal region of human MGAT5. |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein |
| Entrez Gene | 4249 |
|---|---|
| Nucleotide Accession | NP_002401.1 |
Application
MGAT5 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the MGAT5 antibody detected the MGAT5 protein (arrow).
Application
MGAT5 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
