Application
Western blot analysis of MGMT antibody (C-term) in CEM cell line lysates (35ug/lane). MGMT (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human placenta with MGMT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of CEM cells using MGMT Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of MGMT (arrow) using rabbit polyclonal MGMT Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MGMT gene.| Product Name | MGMT Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGMT; Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MGMT Methylated-DNA--protein-cysteine methyltransferase 6-O-methylguanine-DNA methyltransferase O-6-methylguanine-DNA-alkyltransferase |
|---|---|
| Molecular Weight(MW) | 21646 Da |
| Function | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated |
| Tissue Specificity | This MGMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61~90 amino acids from the C-terminal region of human MGMT. |
| Cellular Localization | Nucleus. |
Application
Western blot analysis of MGMT antibody (C-term) in CEM cell line lysates (35ug/lane). MGMT (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human placenta with MGMT Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of CEM cells using MGMT Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of MGMT (arrow) using rabbit polyclonal MGMT Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MGMT gene.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
