Application
MGMT Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).Application
Confocal immunofluorescent analysis of MGMT Antibody (N-term)(Cat#AP13848a) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | MGMT Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGMT; Methylated-DNA--protein-cysteine methyltransferase; 6-O-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MGMT Methylated-DNA--protein-cysteine methyltransferase 6-O-methylguanine-DNA methyltransferase O-6-methylguanine-DNA-alkyltransferase |
|---|---|
| Molecular Weight(MW) | 21646 Da |
| Function | Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated |
| Tissue Specificity | This MGMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human MGMT. |
| Cellular Localization | Nucleus. |
Application
MGMT Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the MGMT antibody detected the MGMT protein (arrow).
Application
Confocal immunofluorescent analysis of MGMT Antibody (N-term)(Cat#AP13848a) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
