Application
MIA40 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MIA40 antibody detected the MIA40 protein (arrow).Application
MIA40 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MIA40 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | MIA40 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHCHD4; MIA40; Mitochondrial intermembrane space import and assembly protein 40; Coiled-coil-helix-coiled-coil-helix domain-containing protein 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MIA40 |
|---|---|
| Alternative Names | CHCHD4 MIA40 Mitochondrial intermembrane space import and assembly protein 40 Coiled-coil-helix-coiled-coil-helix domain-containing protein 4 |
| Molecular Weight(MW) | 15996 Da |
| Function | Functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. Required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS) Precursor proteins to be imported into the IMS are translocated in their reduced form into the mitochondria. The oxidized form of CHCHD4/MIA40 forms a transient intermolecular disulfide bridge with the reduced precursor protein, resulting in oxidation of the precursor protein that now contains an intramolecular disulfide bond and is able to undergo folding in the IMS. Reduced CHCHD4/MIA40 is then reoxidized by GFER/ERV1 via a disulfide relay system |
| Tissue Specificity | Expressed in all tissues tested, suggesting an ubiquitous expression |
| Cellular Localization | Mitochondrion intermembrane space. |
| Entrez Gene | 131474 |
|---|---|
| Nucleotide Accession | NP_001091972.1 NP_653237.1 |
Application
MIA40 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MIA40 antibody detected the MIA40 protein (arrow).
Application
MIA40 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MIA40 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
