Application
Confocal immunofluorescent analysis of MIF Antibody (C-term) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of MIF Antibody (C-term) in MCF-7 cell line lysates (35ug/lane).MIF (arrow) was detected using the purified Pab.Application
MIF Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MIF Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MIF; GLIF; MMIF; Macrophage migration inhibitory factor; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWBFC |
| IF:1:10~50 WB |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GLIF MMIF |
|---|---|
| Alternative Names | MIF GLIF MMIF Macrophage migration inhibitory factor Glycosylation-inhibiting factor L-dopachrome isomerase L-dopachrome tautomerase Phenylpyruvate tautomerase |
| Molecular Weight(MW) | 12476 Da |
| Function | Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti- inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity |
| Tissue Specificity | This MIF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-110 amino acids from the C-terminal region of human MIF. |
| Cellular Localization | Secreted. Cytoplasm. Note=Does not have a cleavable signal sequence and is secreted via a specialized, non- classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens |
| Entrez Gene | 4282 |
|---|
Application
Confocal immunofluorescent analysis of MIF Antibody (C-term) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of MIF Antibody (C-term) in MCF-7 cell line lysates (35ug/lane).MIF (arrow) was detected using the purified Pab.
Application
MIF Antibody (C-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | IF:1:10~50 WB |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Data sheet for OM213727