Application
Western blot analysis of MLK4 (arrow) using rabbit polyclonal MLK4 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KIAA1804 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MLK4 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | MLK4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLK4; KIAA1804; Mitogen-activated protein kinase kinase kinase MLK4; Mixed lineage kinase 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1804 |
|---|---|
| Alternative Names | MLK4 KIAA1804 Mitogen-activated protein kinase kinase kinase MLK4 Mixed lineage kinase 4 |
| Molecular Weight(MW) | 113957 Da |
| Function | Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways |
| Tissue Specificity | This MLK4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40~70 amino acids from the N-terminal region of human MLK4. |
| Entrez Gene | 84451 |
|---|
Application
Western blot analysis of MLK4 (arrow) using rabbit polyclonal MLK4 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KIAA1804 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MLK4 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
