Application
Western blot analysis of anti-MLKLAK-C284 Pab in HepG2 cell line lysate (35ug/lane). MLKLAK-C284(arrow) was detected using the purified Pab (1:60 dilution). This western blot detected isoform 2 of MLKLAK, the accession number for MLKLAK isoform 1 is Q9NYL2, NP_057737.| Product Name | MLKLAK Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLTK; ZAK; Mitogen-activated protein kinase kinase kinase MLT; Human cervical cancer suppressor gene 4 protein; Leucine zipper- and sterile alpha motif-containing kinase; MLK-like mitogen-activated protein triple kinase; Mixed lineage kinase-related kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ZAK |
|---|---|
| Alternative Names | MLTK ZAK Mitogen-activated protein kinase kinase kinase MLT Human cervical cancer suppressor gene 4 protein Leucine zipper- and sterile alpha motif-containing kinase MLK-like mitogen-activated protein triple kinase Mixed lineage kinase-related kinase |
| Molecular Weight(MW) | 91155 Da |
| Function | Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Pro- apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2. Isoform 1, but not isoform 2, causes cell shrinkage and disruption of actin stress fibers Isoform 1 may have role in neoplastic cell transformation and cancer development. Isoform 1, but not isoform 2, phosphorylates histone H3 at 'Ser-28' |
| Tissue Specificity | Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocates to the nucleus upon ultraviolet B irradiation |
Application
Western blot analysis of anti-MLKLAK-C284 Pab in HepG2 cell line lysate (35ug/lane). MLKLAK-C284(arrow) was detected using the purified Pab (1:60 dilution). This western blot detected isoform 2 of MLKLAK, the accession number for MLKLAK isoform 1 is Q9NYL2, NP_057737.| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
