Application
MLL5 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MLL5 antibody detected the MLL5 protein (arrow).| Product Name | MLL5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLL5; KMT2E; Histone-lysine N-methyltransferase MLL5; Lysine N-methyltransferase 2E; Myeloid/lymphoid or mixed-lineage leukemia protein 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KMT2E |
|---|---|
| Alternative Names | MLL5 KMT2E Histone-lysine N-methyltransferase MLL5 Lysine N-methyltransferase 2E Myeloid/lymphoid or mixed-lineage leukemia protein 5 |
| Molecular Weight(MW) | 204965 Da |
| Function | Histone methyltransferase that specifically mono- and dimethylates 'Lys-4' of histone H3 (H3K4me1 and H3K4me2). H3 'Lys- 4' methylation represents a specific tag for epigenetic transcriptional activation. Key regulator of hematopoiesis involved in terminal myeloid differentiation and in the regulation of hematopoietic stem cell (HSCs) self-renewal by a mechanism that involves DNA methylation. Plays an essential role in retinoic- acid-induced granulopoiesis by acting as a coactivator of RAR- alpha (RARA) in target gene promoters. Also acts as an important cell cycle regulator, participating in cell cycle regulatory network machinery at multiple cell cycle stages. Required to suppress inappropriate expression of S-phase-promoting genes and maintain expression of determination genes in quiescent cells Overexpression inhibits cell cycle progression, while knockdown induces cell cycle arrest at both the G1 and G2/M phases |
| Tissue Specificity | Widely expressed in both adult and fetal tissues. Highest levels of expression observed in fetal thymus and kidney and in adult hematopoietic tissues, jejunum and cerebellum |
| Cellular Localization | Nucleus speckle. Note=Absent from the nucleolus |
| Entrez Gene | 55904 |
|---|---|
| Nucleotide Accession | Q8NFF8 NP_891847.1 NP_061152.3 |
Application
MLL5 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MLL5 antibody detected the MLL5 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
