Application
Western blot analysis of MLLT10 in K562 cell line lysates (35ug/lane). MLLT10 (arrow) was detected using the purified Pab.(2ug/ml)Application
Fluorescent confocal image of Hela cell stained with MLLT10 (AF10) Antibody (Center)(Cat#AP1906b).HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MLLT10 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). MLLT10 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.| Product Name | MLLT10 (AF10) Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLLT10; AF10; Protein AF-10; ALL1-fused gene from chromosome 10 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:50~100 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | AF10 |
|---|---|
| Alternative Names | MLLT10 AF10 Protein AF-10 ALL1-fused gene from chromosome 10 protein |
| Molecular Weight(MW) | 109026 Da |
| Function | Probably involved in transcriptional regulation. In vitro or as fusion protein with MLL has transactivation activity Binds to cruciform DNA |
| Tissue Specificity | Expressed abundantly in testis. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 8028 |
|---|
Application
Western blot analysis of MLLT10 in K562 cell line lysates (35ug/lane). MLLT10 (arrow) was detected using the purified Pab.(2ug/ml)
Application
Fluorescent confocal image of Hela cell stained with MLLT10 (AF10) Antibody (Center)(Cat#AP1906b).HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MLLT10 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). MLLT10 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.| Application Notes | WB:1:50~100 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
