Application
Western blot analysis of MLX Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). MLX (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with MLX Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of NCI-H292 cells using MLX Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of MLX (arrow) using rabbit polyclonal MLX Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLX gene.| Product Name | MLX Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MLX; BHLHD13; TCFL4; Max-like protein X; Class D basic helix-loop-helix protein 13; Max-like bHLHZip protein; Protein BigMax; Transcription factor-like protein 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | BHLHD13 TCFL4 |
|---|---|
| Alternative Names | MLX BHLHD13 TCFL4 Max-like protein X Class D basic helix-loop-helix protein 13 Max-like bHLHZip protein Protein BigMax Transcription factor-like protein 4 |
| Molecular Weight(MW) | 33300 Da |
| Function | Transcription regulator. Forms a sequence-specific DNA- binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation |
| Tissue Specificity | Expressed in all tissues tested, including spleen, thymus, prostate, ovary, intestine, colon, peripheral blood leukocyte, heart, liver, skeletal muscle and kidney. Lower levels of expression in testis, brain, placenta and lung |
| Cellular Localization | Isoform Alpha: Cytoplasm. Note=Found predominantly in the cytoplasm Isoform Gamma: Nucleus. Note=Found predominantly in the nucleus |
| Entrez Gene | 6945 |
|---|
Application
Western blot analysis of MLX Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). MLX (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with MLX Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of NCI-H292 cells using MLX Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of MLX (arrow) using rabbit polyclonal MLX Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLX gene.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
