Application
The anti-MMP14 N-term Antibody is used in Western blot to detect MMP14 in A375 lysate.Application
Western blot analysis of MMP14 (arrow) using rabbit polyclonal MMP14 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP14 gene (Lane 2) (Origene Technologies).Application
Flow cytometric analysis of MCF-7 cells using MMP14 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MMP14 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MMP14; Matrix metalloproteinase-14; MMP-X1; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | MMP14 Matrix metalloproteinase-14 MMP-X1 Membrane-type matrix metalloproteinase 1 Membrane-type-1 matrix metalloproteinase |
|---|---|
| Molecular Weight(MW) | 65894 Da |
| Function | Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15 |
| Tissue Specificity | Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors |
| Cellular Localization | Membrane; Single-pass type I membrane protein (Potential). Melanosome. Cytoplasm. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Forms a complex with BST2 and localizes to the cytoplasm |
Application
The anti-MMP14 N-term Antibody is used in Western blot to detect MMP14 in A375 lysate.
Application
Western blot analysis of MMP14 (arrow) using rabbit polyclonal MMP14 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP14 gene (Lane 2) (Origene Technologies).
Application
Flow cytometric analysis of MCF-7 cells using MMP14 Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
