Application
MMP2 western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the MMP2 antibody detected the MMP2 protein (arrow).Application
Confocal immunofluorescent analysis of MMP2 Antibody (Cat#AM1844b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).Application
MMP2 Antibody (Cat. #AM1844b)immunohistochemistry analysis in formalin fixed and paraffin embedded human gastric carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MMP2 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
MMP2 Antibody (Cat. #AM1844b)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MMP2 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | MMP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MMP2; CLG4A; 72 kDa type IV collagenase; 72 kDa gelatinase; Gelatinase A; Matrix metalloproteinase-2; TBE-1; PEX |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2b |
| Clone Number | 6E3F8 |
| Host Species | Mouse |
| Tested Applications | WBIFIHC |
| WB:1:100~250 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CLG4A |
|---|---|
| Alternative Names | MMP2 CLG4A 72 kDa type IV collagenase 72 kDa gelatinase Gelatinase A Matrix metalloproteinase-2 TBE-1 PEX |
| Molecular Weight(MW) | 73882 Da |
| Function | Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro Isoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro- inflammatory NF-kappaB, NFAT and IRF transcriptional pathways |
| Tissue Specificity | Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate |
| Cellular Localization | Isoform 1: Secreted, extracellular space, extracellular matrix. Membrane. Nucleus. Note=Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes |
| Entrez Gene | 4313 |
|---|
Application
MMP2 western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the MMP2 antibody detected the MMP2 protein (arrow).
Application
Confocal immunofluorescent analysis of MMP2 Antibody (Cat#AM1844b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
MMP2 Antibody (Cat. #AM1844b)immunohistochemistry analysis in formalin fixed and paraffin embedded human gastric carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MMP2 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
MMP2 Antibody (Cat. #AM1844b)immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MMP2 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~250 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
