Application
Western blot analysis of MMP3 (arrow) using rabbit polyclonal MMP3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP3 gene (Lane 2) (Origene Technologies).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of HepG2 cells using MMP3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | MMP3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MMP3; STMY1; Stromelysin-1; Matrix metalloproteinase-3; Transin-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | STMY1 |
|---|---|
| Alternative Names | MMP3 STMY1 Stromelysin-1 Matrix metalloproteinase-3 Transin-1 |
| Molecular Weight(MW) | 53977 Da |
| Function | Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase |
| Tissue Specificity | This MMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human MMP3. |
| Cellular Localization | Secreted, extracellular space, extracellular matrix (Probable) |
Application
Western blot analysis of MMP3 (arrow) using rabbit polyclonal MMP3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MMP3 gene (Lane 2) (Origene Technologies).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MMP3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of HepG2 cells using MMP3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
