Application
MOCS3 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MOCS3 antibody detected the MOCS3 protein (arrow).| Product Name | MOCS3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MOCS3; UBA4; Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; Molybdopterin-synthase adenylyltransferase; Adenylyltransferase MOCS3; Sulfur carrier protein MOCS2A adenylyltransfer |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | UBA4 |
|---|---|
| Alternative Names | MOCS3 UBA4 Adenylyltransferase and sulfurtransferase MOCS3 Molybdenum cofactor synthesis protein 3 Molybdopterin synthase sulfurylase Molybdopterin-synthase adenylyltransferase Adenylyltransferase MOCS3 Sulfur carrier protein MOCS2A adenylyltransfer |
| Molecular Weight(MW) | 49669 Da |
| Function | Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed Does not use thiosulfate as sulfur donor; NFS1 probably acting as a sulfur donor for thiocarboxylation reactions |
| Tissue Specificity | This MOCS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-66 amino acids from the N-terminal region of human MOCS3. |
| Cellular Localization | Cytoplasm, cytosol. |
| Entrez Gene | 27304 |
|---|---|
| Nucleotide Accession | NP_055299.1 |
Application
MOCS3 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MOCS3 antibody detected the MOCS3 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
