Application
Mouse Aak1 Antibody (Center) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Aak1 antibody detected the Aak1 protein (arrow).Application
Mouse Aak1 Antibody (Center) (AP14604c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Aak1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Aak1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aak1; Kiaa1048; AP2-associated protein kinase 1; Adaptor-associated kinase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Kiaa1048 |
|---|---|
| Alternative Names | Aak1 Kiaa1048 AP2-associated protein kinase 1 Adaptor-associated kinase 1 |
| Molecular Weight(MW) | 103346 Da |
| Function | Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity (By similarity) |
| Tissue Specificity | This Mouse Aak1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 278-306 amino acids from the Central region of Mouse Aak1. |
| Cellular Localization | Cell membrane; Peripheral membrane protein (By similarity). Membrane, clathrin-coated pit (By similarity) Note=Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells (By similarity) |
| Entrez Gene | 269774 |
|---|---|
| Nucleotide Accession | NP_808430.2 NP_001035195.1 |
Application
Mouse Aak1 Antibody (Center) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Aak1 antibody detected the Aak1 protein (arrow).
Application
Mouse Aak1 Antibody (Center) (AP14604c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Aak1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
