Application
Western blot analysis of anti-mouse BID Antibody (S61) in mouse spleen tissue lysates (35ug/lane). BID (arrow) was detected using the purified Pab .Application
mouse BID Antibody (S61) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse BID Antibody (S61) for immunohistochemistry. Clinical relevance has not been evaluated.Application
mouse BID Antibody (S61) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | BID Antibody (S61) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bid; BH3-interacting domain death agonist; p22 BID; BH3-interacting domain death agonist p15; p15 BID; BH3-interacting domain death agonist p13; p13 BID; BH3-interacting domain death agonist p11; p11 BID |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Bid BH3-interacting domain death agonist p22 BID BH3-interacting domain death agonist p15 p15 BID BH3-interacting domain death agonist p13 p13 BID BH3-interacting domain death agonist p11 p11 BID |
|---|---|
| Molecular Weight(MW) | 21952 Da |
| Function | Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c |
| Tissue Specificity | This mouse BID antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S61 of mouse BID. |
| Cellular Localization | Cytoplasm. Mitochondrion membrane. Note=When uncleaved, it is predominantly cytoplasmic. p15 BID translocates to mitochondria as an integral membrane protein. p13 and p22 BID are associated with the mitochondrial membrane |
Application
Western blot analysis of anti-mouse BID Antibody (S61) in mouse spleen tissue lysates (35ug/lane). BID (arrow) was detected using the purified Pab .
Application
mouse BID Antibody (S61) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the mouse BID Antibody (S61) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
mouse BID Antibody (S61) FC analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
