Application
Mouse Brsk2 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Brsk2 antibody detected the Brsk2 protein (arrow).| Product Name | Brsk2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Brsk2; Kiaa4256; Sada; Serine/threonine-protein kinase BRSK2; Brain-specific serine/threonine-protein kinase 2; Serine/threonine-protein kinase SAD-A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Kiaa4256 Sada |
|---|---|
| Alternative Names | Brsk2 Kiaa4256 Sada Serine/threonine-protein kinase BRSK2 Brain-specific serine/threonine-protein kinase 2 Serine/threonine-protein kinase SAD-A |
| Molecular Weight(MW) | 81733 Da |
| Function | Serine/threonine-protein kinase that plays a key role in polarization of neurons and axonogenesis, cell cycle progress and insulin secretion. Phosphorylates CDK16, CDC25C, MAPT/TAU, PAK1 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-504' and 'Ser-554'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. Plays a role in the regulation of the mitotic cell cycle progress and the onset of mitosis. Plays a role in the regulation of insulin secretion in response to elevated glucose levels, probably via phosphorylation of CDK16 and PAK1. While BRSK2 phosphorylated at Thr-175 can inhibit insulin secretion (PubMed:22798068), BRSK2 phosphorylated at Thr-261 can promote insulin secretion (PubMed:22669945). Regulates reorganization of the actin cytoskeleton. May play a role in the apoptotic response triggered by endoplasmatic reticulum (ER) stress |
| Tissue Specificity | Detected in pancreas islets and in brain (at protein level). Detected in brain and pancreas |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome (By similarity). Cytoplasm, perinuclear region (By similarity) Endoplasmic reticulum (By similarity). Note=Detected at centrosomes during mitosis. Localizes to the endoplasmic reticulum in response to stress caused by tunicamycin (By similarity) |
| Entrez Gene | 75770 |
|---|---|
| Nucleotide Accession | NP_001009930.1 NP_083702.1 |
Application
Mouse Brsk2 Antibody (Center) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the Brsk2 antibody detected the Brsk2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
