Application
Mouse Camk2d Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Camk2d antibody detected the Camk2d protein (arrow).Application
Western blot analysis of Camk2d (arrow) using rabbit polyclonal Mouse Camk2d Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Camk2d gene.Application
Mouse Camk2d Antibody (Center) (AP13799c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Camk2d Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Camk2d Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Camk2d; Kiaa4163; Calcium/calmodulin-dependent protein kinase type II subunit delta |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Kiaa4163 |
|---|---|
| Alternative Names | Camk2d Kiaa4163 Calcium/calmodulin-dependent protein kinase type II subunit delta |
| Molecular Weight(MW) | 56369 Da |
| Function | Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+) channel on 'Ser- 2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca(2+) influx to myocytes by binding and phosphorylating the L-type Ca(2+) channel subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca(2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca(2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor- coupling factor |
| Tissue Specificity | Expressed in cardiac muscle and skeletal muscle. Isoform Delta 2, isoform Delta 6, isoform Delta 6 and isoform Delta 10 are expressed in cardiac muscle. Isoform Delta 2 is expressed in skeletal muscle |
| Cellular Localization | Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side (Probable). Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side (Probable) |
| Entrez Gene | 108058 |
|---|---|
| Nucleotide Accession | NP_001020609.1 NP_076302.1 |
Application
Mouse Camk2d Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Camk2d antibody detected the Camk2d protein (arrow).
Application
Western blot analysis of Camk2d (arrow) using rabbit polyclonal Mouse Camk2d Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Camk2d gene.
Application
Mouse Camk2d Antibody (Center) (AP13799c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Camk2d Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
