Application
Mouse Casc5 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Casc5 antibody detected the Casc5 protein (arrow).| Product Name | Casc5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Casc5; Knl1; Protein CASC5; Cancer susceptibility candidate gene 5 protein homolog; Kinetochore-null protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Knl1 |
|---|---|
| Alternative Names | Casc5 Knl1 Protein CASC5 Cancer susceptibility candidate gene 5 protein homolog Kinetochore-null protein 1 |
| Molecular Weight(MW) | 179337 Da |
| Function | Performs two crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Directly links spindle checkpoint proteins BUB1 and BUB1B to kinetochores. Part of the MIS12 complex, which may be fundamental for kinetochore formation and proper chromosome segregation during mitosis. Acts in coordination with CENPK to recruit the NDC80 complex to the outer kinetochore (By similarity) |
| Tissue Specificity | This Mouse Casc5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1556-1583 amino acids from the C-terminal region of mouse Casc5. |
| Cellular Localization | Nucleus (By similarity). Chromosome, centromere, kinetochore (By similarity). Note=Weakly expressed in interphase nuclei. Expression increases from prophase to late anaphase, but greatly diminishes from the telophase and cytokinesis to early G1 phase of cell cycle (By similarity) |
Application
Mouse Casc5 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Casc5 antibody detected the Casc5 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
