Application
Mouse CASP3 Antibody (N-term S12) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the CASP3 antibody detected the CASP3 protein (arrow).Application
mouse CASP3 Antibody (S12) western blot analysis in rat cerebellum tissue lysates (35ug/lane).This demonstrates the CASP3 antibody detected the CASP3 protein (arrow).| Product Name | CASP3 Antibody (N-term S12) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Casp3; Cpp32; Caspase-3; Apopain; Cysteine protease CPP32; LICE; Protein Yama; SREBP cleavage activity 1; Caspase-3 subunit p17; Caspase-3 subunit p12 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Cpp32 |
|---|---|
| Alternative Names | Casp3 Cpp32 Caspase-3 Apopain Cysteine protease CPP32 LICE Protein Yama SREBP cleavage activity 1 Caspase-3 subunit p17 Caspase-3 subunit p12 |
| Molecular Weight(MW) | 31475 Da |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity) Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes |
| Tissue Specificity | Highest expression in spleen, lung, liver, kidney and heart. Lower expression in brain, skeletal muscle and testis |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 12367 |
|---|---|
| Nucleotide Accession | NP_033940.1 |
Application
Mouse CASP3 Antibody (N-term S12) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the CASP3 antibody detected the CASP3 protein (arrow).
Application
mouse CASP3 Antibody (S12) western blot analysis in rat cerebellum tissue lysates (35ug/lane).This demonstrates the CASP3 antibody detected the CASP3 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
