Application
Dot blot analysis of Phospho-mouse CASP3-S26 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.| Product Name | Phospho-mouse CASP3-S26 |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-S26 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | DB |
| DB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Cpp32 |
|---|---|
| Alternative Names | Casp3 Cpp32 Caspase-3 Apopain Cysteine protease CPP32 LICE Protein Yama SREBP cleavage activity 1 Caspase-3 subunit p17 Caspase-3 subunit p12 |
| Molecular Weight(MW) | 31475 Da |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity) Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes |
| Tissue Specificity | Highest expression in spleen, lung, liver, kidney and heart. Lower expression in brain, skeletal muscle and testis |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 12367 |
|---|---|
| Nucleotide Accession | NP_033940.1 |
Application
Dot blot analysis of Phospho-mouse CASP3-S26 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.| Application Notes | DB:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by two-step phosphospecific peptide affinity purification. |
