Application
Mouse Cblb Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Cblb antibody detected the Cblb protein (arrow).| Product Name | Cblb Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cblb; E3 ubiquitin-protein ligase CBL-B; Casitas B-lineage lymphoma proto-oncogene b; SH3-binding protein CBL-B; Signal transduction protein CBL-B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Cblb E3 ubiquitin-protein ligase CBL-B Casitas B-lineage lymphoma proto-oncogene b SH3-binding protein CBL-B Signal transduction protein CBL-B |
|---|---|
| Molecular Weight(MW) | 109092 Da |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B- cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T- cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization |
| Tissue Specificity | This Mouse Cblb antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 861-889 amino acids from the C-terminal region of mouse Cblb. |
| Cellular Localization | Cytoplasm. Note=In adipocytes, translocates to the plasma membrane upon insulin stimulation |
| Entrez Gene | 208650 |
|---|---|
| Nucleotide Accession | NP_001028410.1 |
Application
Mouse Cblb Antibody (C-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Cblb antibody detected the Cblb protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
