Application
Mouse Cdk4 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Cdk4 antibody detected the Cdk4 protein (arrow).| Product Name | Cdk4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cdk4; Crk3; Cyclin-dependent kinase 4; CRK3; Cell division protein kinase 4; PSK-J3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Crk3 |
|---|---|
| Alternative Names | Cdk4 Crk3 Cyclin-dependent kinase 4 CRK3 Cell division protein kinase 4 PSK-J3 |
| Molecular Weight(MW) | 33751 Da |
| Function | Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity) |
| Tissue Specificity | This Mouse Cdk4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 218-246 amino acids from the C-terminal region of Mouse Cdk4. |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity). Membrane (By similarity). Note=Cytoplasmic when non- complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps Colocalizes with RB1 after release into the nucleus (By similarity) |
| Entrez Gene | 12567 |
|---|---|
| Nucleotide Accession | NP_034000.1 |
Application
Mouse Cdk4 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Cdk4 antibody detected the Cdk4 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
