Application
Mouse Cdkn2a Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Cdkn2a antibody detected the Cdkn2a protein (arrow).| Product Name | Cdkn2a Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cdkn2a; Cyclin-dependent kinase inhibitor 2A, isoform 3; p19ARF |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Cdkn2a Cyclin-dependent kinase inhibitor 2A isoform 3 p19ARF |
|---|---|
| Molecular Weight(MW) | 19238 Da |
| Function | Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus This inhibits the oncogenic action of MDM2 by blocking MDM2- induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination (By similarity). Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform 4 may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP |
| Tissue Specificity | This Mouse Cdkn2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-65 amino acids from the Central region of mouse Cdkn2a. |
| Cellular Localization | Nucleus, nucleolus. Nucleus, nucleoplasm (By similarity) |
| Entrez Gene | 12578 |
|---|---|
| Nucleotide Accession | NP_034007.1 |
Application
Mouse Cdkn2a Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Cdkn2a antibody detected the Cdkn2a protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
