Application
Mouse Chek2 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Chek2 antibody detected the Chek2 protein (arrow).Application
Mouse Chek2 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Chek2 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Chek2; Chk2; Rad53; Serine/threonine-protein kinase Chk2; CHK2 checkpoint homolog; Checkpoint kinase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Chk2 Rad53 |
|---|---|
| Alternative Names | Chek2 Chk2 Rad53 Serine/threonine-protein kinase Chk2 CHK2 checkpoint homolog Checkpoint kinase 2 |
| Molecular Weight(MW) | 61088 Da |
| Function | Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X- R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1 Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells |
| Tissue Specificity | Ubiquitously expressed with higher levels in the thymus, spleen and colon (at protein level) |
| Cellular Localization | Nucleus, PML body (By similarity). Nucleus, nucleoplasm. Note=Recruited into PML bodies together with TP53 (By similarity) |
| Entrez Gene | 50883 |
|---|---|
| Nucleotide Accession | NP_057890.1 |
Application
Mouse Chek2 Antibody (C-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Chek2 antibody detected the Chek2 protein (arrow).
Application
Mouse Chek2 Antibody (C-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
