Application
Western blot analysis of DHFR antibody (C-term) (Cat.# AP7378b) in mouse liver tissue lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab.Application
DHFR Antibody (C-term) (Cat.# AP7378b) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHFR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of DHFR (arrow) using rabbit polyclonal DHFR Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DHFR gene.| Product Name | DHFR Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Dhfr; Dihydrofolate reductase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Dhfr Dihydrofolate reductase |
|---|---|
| Molecular Weight(MW) | 21606 Da |
| Function | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFRL1 |
| Tissue Specificity | This Mouse DHFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142~172 amino acids from the C-terminal region of mouse DHFR. |
| Entrez Gene | 13361 |
|---|
Application
Western blot analysis of DHFR antibody (C-term) (Cat.# AP7378b) in mouse liver tissue lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab.
Application
DHFR Antibody (C-term) (Cat.# AP7378b) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DHFR Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of DHFR (arrow) using rabbit polyclonal DHFR Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DHFR gene.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
