Application
Western blot analysis of anti-mDnmt2 Pab in HL-60 cell lysate. mDnmt2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | Dnmt2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Trdmt1; Dnmt2; Met2; tRNA (cytosine(38)-C(5))-methyltransferase; DNA (cytosine-5)-methyltransferase-like protein 2; DNA methyltransferase homolog MmuIIP; Met-2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Dnmt2 Met2 |
|---|---|
| Alternative Names | Trdmt1 Dnmt2 Met2 tRNA (cytosine(38)-C(5))-methyltransferase DNA (cytosine-5)-methyltransferase-like protein 2 DNA methyltransferase homolog MmuIIP Met-2 |
| Molecular Weight(MW) | 46794 Da |
| Function | Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp) (By similarity) |
| Tissue Specificity | Highly expressed in thymus, testis, and at much lower levels in spleen, lung, brain, heart, kidney, liver, skeletal muscle and embryonic stem cells |
| Cellular Localization | Nucleus (Probable). |
| Entrez Gene | 13434 |
|---|
Application
Western blot analysis of anti-mDnmt2 Pab in HL-60 cell lysate. mDnmt2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
