Application
Mouse Eif2ak1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Eif2ak1 antibody detected the Eif2ak1 protein (arrow).Application
Mouse Eif2ak1 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Eif2ak1 antibody detected the Eif2ak1 protein (arrow).Application
Mouse Eif2ak1 Antibody (N-term) (AP13913a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Eif2ak1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Eif2ak1 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Eif2ak1; Hri; Eukaryotic translation initiation factor 2-alpha kinase 1; Heme-controlled repressor; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2-alpha kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Hri |
|---|---|
| Alternative Names | Eif2ak1 Hri Eukaryotic translation initiation factor 2-alpha kinase 1 Heme-controlled repressor Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase Heme-regulated inhibitor Hemin-sensitive initiation factor 2-alpha kinase |
| Molecular Weight(MW) | 69702 Da |
| Function | Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock Exerts its function through the phosphorylation of EIF2S1 at 'Ser- 48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions |
| Tissue Specificity | Expressed predominantly in erythroid cells, mature reticulocytes, as well as fetal liver nucleated erythroid cells. At much lower levels, expressed in hepatocytes and bone marrow-derived macrophages (at protein level) |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 15467 |
|---|
Application
Mouse Eif2ak1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Eif2ak1 antibody detected the Eif2ak1 protein (arrow).
Application
Mouse Eif2ak1 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Eif2ak1 antibody detected the Eif2ak1 protein (arrow).
Application
Mouse Eif2ak1 Antibody (N-term) (AP13913a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Eif2ak1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
