Application
Mouse Ephb3 Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the Ephb3 antibody detected the Ephb3 protein (arrow).Application
Mouse Ephb3 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Ephb3 antibody detected the Ephb3 protein (arrow).Application
Mouse Ephb3 Antibody (Center) (AP14819c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Ephb3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Ephb3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ephb3; Etk2; Mdk5; Sek4; Ephrin type-B receptor 3; Developmental kinase 5; Tyrosine-protein kinase receptor SEK-4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Etk2 Mdk5 Sek4 |
|---|---|
| Alternative Names | Ephb3 Etk2 Mdk5 Sek4 Ephrin type-B receptor 3 Developmental kinase 5 Tyrosine-protein kinase receptor SEK-4 |
| Molecular Weight(MW) | 109662 Da |
| Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex Beside its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt |
| Tissue Specificity | Expressed in cells of the retinal ganglion cell layer during retinal axon guidance to the optic disk Expressed by Paneth and progenitor cells in the crypts of the intestinal epithelium (at protein level) |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Cell projection, dendrite |
| Entrez Gene | 13845 |
|---|
Application
Mouse Ephb3 Antibody (Center) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the Ephb3 antibody detected the Ephb3 protein (arrow).
Application
Mouse Ephb3 Antibody (Center) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Ephb3 antibody detected the Ephb3 protein (arrow).
Application
Mouse Ephb3 Antibody (Center) (AP14819c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Ephb3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
