Application
Mouse Fgfr1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Fgfr1 antibody detected the Fgfr1 protein (arrow).Application
Mouse Fgfr1 Antibody (C-term) (AP14840b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse adrenal glands followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Fgfr1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Fgfr1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Fgfr1; Flg; Fibroblast growth factor receptor 1; Basic fibroblast growth factor receptor 1; MFR; Proto-oncogene c-Fgr; CD_antigen=CD331; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Flg |
|---|---|
| Alternative Names | Fgfr1 Flg Fibroblast growth factor receptor 1 Basic fibroblast growth factor receptor 1 MFR Proto-oncogene c-Fgr CD_antigen=CD331 Flags: Precursor |
| Molecular Weight(MW) | 91981 Da |
| Function | Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system Phosphorylates PLCG1, FRS2, GAB1 and SHB. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes phosphorylation of SHC1, STAT1 and PTPN11/SHP2 In the nucleus, enhances RPS6KA1 and CREB1 activity and contributes to the regulation of transcription. FGFR1 signaling is down-regulated by IL17RD/SEF, and by FGFR1 ubiquitination, internalization and degradation (By similarity) |
| Tissue Specificity | Widely expressed. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Nucleus. Cytoplasm, cytosol. Cytoplasmic vesicle Note=After ligand binding, both receptor and ligand are rapidly internalized. Can translocate to the nucleus after internalization, or by translocation from the endoplasmic reticulum or Golgi apparatus to the cytosol, and from there to the nucleus Isoform 5: Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 14182 |
|---|---|
| Nucleotide Accession | NP_001073378.1 NP_001073377.1 |
Application
Mouse Fgfr1 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Fgfr1 antibody detected the Fgfr1 protein (arrow).
Application
Mouse Fgfr1 Antibody (C-term) (AP14840b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse adrenal glands followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Fgfr1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
