Application
Flt4 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the Flt4 antibody detected the Flt4 protein (arrow).Application
Flt4 Antibody (N-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the Flt4 antibody detected the Flt4 protein (arrow).Application
Flt4 Antibody (N-term) (AP14163a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Flt4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Flt4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Flt4; Flt-4; Vegfr3; Vascular endothelial growth factor receptor 3; Fms-like tyrosine kinase 4; Tyrosine-protein kinase receptor FLT4 |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500~1:2000 IHC:1:50~1:200 IF:100~1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Flt-4 Vegfr3 |
|---|---|
| Alternative Names | Flt4 Flt-4 Vegfr3 Vascular endothelial growth factor receptor 3 Fms-like tyrosine kinase 4 Tyrosine-protein kinase receptor FLT4 |
| Molecular Weight(MW) | 153016 Da |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFC and VEGFD, and plays an essential role in adult lymphangiogenesis and in the development of the vascular network and the cardiovascular system during embryonic development Promotes proliferation, survival and migration of endothelial cells, and regulates angiogenic sprouting. Signaling by activated FLT4 leads to enhanced production of VEGFC, and to a lesser degree VEGFA, thereby creating a positive feedback loop that enhances FLT4 signaling. Modulates KDR signaling by forming heterodimers Mediates activation of the MAPK1/ERK2, MAPK3/ERK1 signaling pathway, of MAPK8 and the JUN signaling pathway, and of the AKT1 signaling pathway. Phosphorylates SHC1. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3- kinase. Promotes phosphorylation of MAPK8 at 'Thr-183' and 'Tyr- 185', and of AKT1 at 'Ser-473' |
| Tissue Specificity | Expressed in adult lung and liver, and in fetal liver, brain, intestine and placenta |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Cytoplasm. Nucleus (By similarity). Note=Ligand-mediated autophosphorylation leads to rapid internalization |
| Entrez Gene | 14257 |
|---|---|
| Nucleotide Accession | NP_032055.1 |
Application
Flt4 Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the Flt4 antibody detected the Flt4 protein (arrow).
Application
Flt4 Antibody (N-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the Flt4 antibody detected the Flt4 protein (arrow).
Application
Flt4 Antibody (N-term) (AP14163a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Flt4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:500~1:2000 IHC:1:50~1:200 IF:100~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
