Application
Mouse Gsk3a Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Gsk3a antibody detected the Gsk3a protein (arrow).Application
Mouse Gsk3a Antibody (C-term) (AP14846b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse lymph node(mandibular) followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Gsk3a Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Gsk3a Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gsk3a; Glycogen synthase kinase-3 alpha; Serine/threonine-protein kinase GSK3A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Gsk3a Glycogen synthase kinase-3 alpha Serine/threonine-protein kinase GSK3A |
|---|---|
| Molecular Weight(MW) | 51661 Da |
| Function | Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1 Requires primed phosphorylation of the majority of its substrates Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth |
| Tissue Specificity | This Mouse Gsk3a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 347-376 amino acids from the C-terminal region of Mouse Gsk3a. |
| Entrez Gene | 606496 |
|---|---|
| Nucleotide Accession | NP_001026837.1 |
Application
Mouse Gsk3a Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Gsk3a antibody detected the Gsk3a protein (arrow).
Application
Mouse Gsk3a Antibody (C-term) (AP14846b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse lymph node(mandibular) followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Gsk3a Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
