Application
Mouse Jak3 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Jak3 antibody detected the Jak3 protein (arrow).Application
Mouse Jak3 Antibody (N-term) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the Jak3 antibody detected the Jak3 protein (arrow).| Product Name | Jak3 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Jak3; Tyrosine-protein kinase JAK3; Janus kinase 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Jak3 Tyrosine-protein kinase JAK3 Janus kinase 3 |
|---|---|
| Molecular Weight(MW) | 122641 Da |
| Function | Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hematopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the cytoplasmic tails of the receptor, creating docking sites for STATs proteins Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion |
| Tissue Specificity | In contrast with the ubiquitous expression of the other JAKs, JAK3 is predominantly expressed in hematopoietic tissues |
| Cellular Localization | Endomembrane system; Peripheral membrane protein (By similarity). Cytoplasm (By similarity) |
| Entrez Gene | 16453 |
|---|---|
| Nucleotide Accession | NP_034719.2 NP_001177759.1 |
Application
Mouse Jak3 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the Jak3 antibody detected the Jak3 protein (arrow).
Application
Mouse Jak3 Antibody (N-term) western blot analysis in mouse stomach tissue lysates (35ug/lane).This demonstrates the Jak3 antibody detected the Jak3 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
