Application
Mouse Irak1 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Irak1 antibody detected the Irak1 protein (arrow).Application
Mouse Irak1 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Irak1 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Irak1; Il1rak; Interleukin-1 receptor-associated kinase 1; Pelle-like protein kinase |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Il1rak |
|---|---|
| Alternative Names | Irak1 Il1rak Interleukin-1 receptor-associated kinase 1 Pelle-like protein kinase |
| Molecular Weight(MW) | 77269 Da |
| Function | Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor- signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3 (By similarity) |
| Tissue Specificity | Highly expressed in liver, followed by kidney and skeletal muscle |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity). Lipid droplet. Note=Translocates to the nucleus when sumoylated (By similarity). RSAD2/viperin recruits it to the lipid droplet |
| Entrez Gene | 16179 |
|---|---|
| Nucleotide Accession | NP_001171447.1 NP_032389.2 |
Application
Mouse Irak1 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Irak1 antibody detected the Irak1 protein (arrow).
Application
Mouse Irak1 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
