Application
Western blot analysis of anti-JMJD3 C-term Pab in untransfected 293 cells (left) and transfected 293 cells (right). The detection of a prominent band at 180kDa is observed on transfected 293 cells but not on the untransfected 293 cells. Data kindly provided by Dr.Gioacchino Natoli of European Institute of Oncology.Application
Formalin-fixed and paraffin-embedded human Skeletal Muscle tissue reacted with JMJD3 Antibody(C-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | JMJD3 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Kdm6b; Jmjd3; Kiaa0346; Lysine-specific demethylase 6B; JmjC domain-containing protein 3; Jumonji domain-containing protein 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Jmjd3 Kiaa0346 |
|---|---|
| Alternative Names | Kdm6b Jmjd3 Kiaa0346 Lysine-specific demethylase 6B JmjC domain-containing protein 3 Jumonji domain-containing protein 3 |
| Molecular Weight(MW) | 176355 Da |
| Function | Histone demethylase that specifically demethylates 'Lys- 27' of histone H3, thereby playing a central role in histone code Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation |
| Tissue Specificity | This Mouse JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1612~1641 amino acids from the C-terminal region of mouse JMJD3. |
| Cellular Localization | Nucleus (By similarity). |
| Entrez Gene | 216850 |
|---|
Application
Western blot analysis of anti-JMJD3 C-term Pab in untransfected 293 cells (left) and transfected 293 cells (right). The detection of a prominent band at 180kDa is observed on transfected 293 cells but not on the untransfected 293 cells. Data kindly provided by Dr.Gioacchino Natoli of European Institute of Oncology.
Application
Formalin-fixed and paraffin-embedded human Skeletal Muscle tissue reacted with JMJD3 Antibody(C-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
