Application
Mouse Map2k4 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Map2k4 antibody detected the Map2k4 protein (arrow).Application
Mouse Map2k4 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the Map2k4 antibody detected the Map2k4 protein (arrow).Application
Mouse Map2k4 Antibody (C-term) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the Mouse Map2k4 antibody detected the Mouse Map2k4 protein (arrow).Application
Mouse Map2k4 Antibody (C-term) (AP14920b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map2k4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Map2k4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Map2k4; Jnkk1; Mek4; Mkk4; Prkmk4; Sek1; Serk1; Skk1; Dual specificity mitogen-activated protein kinase kinase 4; C-JUN N-terminal kinase kinase 1; JNK-activating kinase 1; MAPK/ERK kinase 4; SAPK/ERK kinase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Jnkk1 Mek4 Mkk4 Prkmk4 Sek1 Serk1 |
|---|---|
| Alternative Names | Map2k4 Jnkk1 Mek4 Mkk4 Prkmk4 Sek1 Serk1 Skk1 Dual specificity mitogen-activated protein kinase kinase 4 C-JUN N-terminal kinase kinase 1 JNK-activating kinase 1 MAPK/ERK kinase 4 SAPK/ERK kinase 1 |
| Molecular Weight(MW) | 44114 Da |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 seems to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14 |
| Tissue Specificity | Strong expression is detected in most of the central nervous system and in liver and thymus during early stages of development. While expression in nervous system increases over time, expression in fetal liver and thymus gradually decreases as embryogenesis proceeds. High level of expression in the central nervous system persists throughout postnatal development and remained at a stable level in adult brain |
| Cellular Localization | Cytoplasm. Nucleus. |
| Entrez Gene | 26398 |
|---|---|
| Nucleotide Accession | NP_033183.1 |
Application
Mouse Map2k4 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Map2k4 antibody detected the Map2k4 protein (arrow).
Application
Mouse Map2k4 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the Map2k4 antibody detected the Map2k4 protein (arrow).
Application
Mouse Map2k4 Antibody (C-term) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the Mouse Map2k4 antibody detected the Mouse Map2k4 protein (arrow).
Application
Mouse Map2k4 Antibody (C-term) (AP14920b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map2k4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
