Application
Mouse Map2k6 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Map2k6 antibody detected the Map2k6 protein (arrow).| Product Name | Map2k6 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Map2k6; Prkmk6; Sapkk3; Dual specificity mitogen-activated protein kinase kinase 6; MAPK/ERK kinase 6; SAPKK3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Prkmk6 Sapkk3 |
|---|---|
| Alternative Names | Map2k6 Prkmk6 Sapkk3 Dual specificity mitogen-activated protein kinase kinase 6 MAPK/ERK kinase 6 SAPKK3 |
| Molecular Weight(MW) | 37432 Da |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG- dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases |
| Tissue Specificity | This Mouse Map2k6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of Mouse Map2k6. |
| Cellular Localization | Nucleus (By similarity). Cytoplasm (By similarity). Cytoplasm, cytoskeleton (By similarity). Note=Binds to microtubules (By similarity) |
| Entrez Gene | 26399 |
|---|---|
| Nucleotide Accession | NP_036073.1 |
Application
Mouse Map2k6 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Map2k6 antibody detected the Map2k6 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
