Application
Mouse Map2k7 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the Map2k7 antibody detected the Map2k7 protein (arrow).Application
Mouse Map2k7 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Map2k7 antibody detected the Map2k7 protein (arrow).Application
Mouse Map2k7 Antibody (Center) (AP14155c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map2k7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Map2k7 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Map2k7; Mkk7; Dual specificity mitogen-activated protein kinase kinase 7; JNK-activating kinase 2; MAPK/ERK kinase 7; c-Jun N-terminal kinase kinase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Mkk7 |
|---|---|
| Alternative Names | Map2k7 Mkk7 Dual specificity mitogen-activated protein kinase kinase 7 JNK-activating kinase 2 MAPK/ERK kinase 7 c-Jun N-terminal kinase kinase 2 |
| Molecular Weight(MW) | 59312 Da |
| Function | Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by proinflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis |
| Tissue Specificity | Expressed at high levels in brain, lung, liver, skeletal muscle, kidney, and testis and at lower levels in the heart and spleen |
| Cellular Localization | Nucleus. Cytoplasm. |
| Entrez Gene | 26400 |
|---|---|
| Nucleotide Accession | NP_001157644.1 NP_001036022.1 |
Application
Mouse Map2k7 Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the Map2k7 antibody detected the Map2k7 protein (arrow).
Application
Mouse Map2k7 Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Map2k7 antibody detected the Map2k7 protein (arrow).
Application
Mouse Map2k7 Antibody (Center) (AP14155c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map2k7 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
