Application
Western blot analysis of Map3k2 (arrow) using rabbit polyclonal Mouse Map3k2 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k2 gene.Application
Mouse Map3k2 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Map3k2 antibody detected the Map3k2 protein (arrow).Application
Mouse Map3k2 (N-term) (AP13917a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map3k2 (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Map3k2 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Map3k2; Mekk2; Mitogen-activated protein kinase kinase kinase 2; MAPK/ERK kinase kinase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Mekk2 |
|---|---|
| Alternative Names | Map3k2 Mekk2 Mitogen-activated protein kinase kinase kinase 2 MAPK/ERK kinase kinase 2 |
| Molecular Weight(MW) | 69574 Da |
| Function | Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7. Plays a role in caveolae kiss- and-run dynamics (By similarity) |
| Tissue Specificity | This Mouse Map3k2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-43 amino acids from the N-terminal region of Mouse Map3k2. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Upon EGF stimulation, translocates into the nucleus |
Application
Western blot analysis of Map3k2 (arrow) using rabbit polyclonal Mouse Map3k2 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k2 gene.
Application
Mouse Map3k2 Antibody (N-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Map3k2 antibody detected the Map3k2 protein (arrow).
Application
Mouse Map3k2 (N-term) (AP13917a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map3k2 (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
