Application
Western blot analysis of Map3k5 (arrow) using rabbit polyclonal Mouse Map3k5 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k5 gene.Application
Mouse Map3k5 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Map3k5 antibody detected the Map3k5 protein (arrow).Application
Mouse Map3k5 Antibody(C-term) (AP14442b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map3k5 Antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Map3k5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Map3k5; Ask1; Mekk5; Mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; MAPK/ERK kinase kinase 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Ask1 Mekk5 |
|---|---|
| Alternative Names | Map3k5 Ask1 Mekk5 Mitogen-activated protein kinase kinase kinase 5 Apoptosis signal-regulating kinase 1 MAPK/ERK kinase kinase 5 |
| Molecular Weight(MW) | 154512 Da |
| Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1) |
| Tissue Specificity | Expressed in various adult mouse tissues including heart, brain, lung, liver and kidney |
| Cellular Localization | Cytoplasm (By similarity). Endoplasmic reticulum (By similarity). Note=Interaction with 14-3-3 proteins alters the distribution of MAP3K5/ASK1 and restricts it to the perinuclear endoplasmic reticulum region (By similarity) |
| Entrez Gene | 26408 |
|---|
Application
Western blot analysis of Map3k5 (arrow) using rabbit polyclonal Mouse Map3k5 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k5 gene.
Application
Mouse Map3k5 Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Map3k5 antibody detected the Map3k5 protein (arrow).
Application
Mouse Map3k5 Antibody(C-term) (AP14442b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map3k5 Antibody(C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
