Application
Mouse Map3k7 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Map3k7 antibody detected the Map3k7 protein (arrow).Application
Western blot analysis of Map3k7 (arrow) using rabbit polyclonal Mouse Map3k7 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k7 gene.Application
Mouse Map3k7 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Map3k7 antibody detected the Map3k7 protein (arrow).Application
Mouse Map3k7 Antibody (N-term) (AP13919a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map3k7 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Map3k7 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Map3k7; Tak1; Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Tak1 |
|---|---|
| Alternative Names | Map3k7 Tak1 Mitogen-activated protein kinase kinase kinase 7 Transforming growth factor-beta-activated kinase 1 |
| Molecular Weight(MW) | 64228 Da |
| Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signal transduction of TRAF6, various cytokines including interleukin-1 (IL-1), transforming growth factor-beta (TGFB), TGFB-related factors like BMP2 and BMP4, toll-like receptors (TLR), tumor necrosis factor receptor CD40 and B-cell receptor (BCR). Ceramides are also able to activate MAP3K7/TAK1. Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K1/MEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs, c-jun N-terminal kinases (JNKs) and I-kappa-B kinase complex (IKK). Both p38 MAPK and JNK pathways control the transcription factors activator protein-1 (AP-1), while nuclear factor-kappa B is activated by IKK. MAP3K7 activates also IKBKB and MAPK8/JNK1 in response to TRAF6 signaling and mediates BMP2- induced apoptosis. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK1, but not that of NF-kappa-B Promotes TRIM5 capsid-specific restriction activity (By similarity) |
| Tissue Specificity | This Mouse Map3k7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-160 amino acids from the N-terminal region of Mouse Map3k7. |
| Cellular Localization | Cytoplasm (By similarity). Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) Note=Although the majority of MAP3K7/TAK1 is found in the cytosol, when complexed with TAB1/MAP3K7IP1 and TAB2/MAP3K7IP2, it is also localized at the cell membrane (By similarity) |
| Entrez Gene | 26409 |
|---|---|
| Nucleotide Accession | NP_766276.1 |
Application
Mouse Map3k7 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Map3k7 antibody detected the Map3k7 protein (arrow).
Application
Western blot analysis of Map3k7 (arrow) using rabbit polyclonal Mouse Map3k7 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k7 gene.
Application
Mouse Map3k7 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Map3k7 antibody detected the Map3k7 protein (arrow).
Application
Mouse Map3k7 Antibody (N-term) (AP13919a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map3k7 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
