Application
Mouse Mapk4 Antibody (N-term) western blot analysis in C6 cell line lysates (35ug/lane).This demonstrates the Mapk4 antibody detected the Mapk4 protein (arrow).Application
Mouse Mapk4 Antibody (N-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the Mapk4 antibody detected the Mapk4 protein (arrow).Application
Mouse Mapk4 Antibody (N-term) (AP14445a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Mapk4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mapk4; Erk4; Prkm4; Mitogen-activated protein kinase 4; Extracellular signal-regulated kinase 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Erk4 Prkm4 |
|---|---|
| Alternative Names | Mapk4 Erk4 Prkm4 Mitogen-activated protein kinase 4 Extracellular signal-regulated kinase 4 |
| Molecular Weight(MW) | 65574 Da |
| Function | Atypical MAPK protein. Phosphorylates microtubule- associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK4/MAPK4 is phosphorylated at Ser-186 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK4/MAPK4. May promote entry in the cell cycle |
| Tissue Specificity | This Mouse Mapk4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of Mouse Mapk4. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following interaction with MAPKAPK5 |
| Entrez Gene | 225724 |
|---|---|
| Nucleotide Accession | NP_766220.2 |
Application
Mouse Mapk4 Antibody (N-term) western blot analysis in C6 cell line lysates (35ug/lane).This demonstrates the Mapk4 antibody detected the Mapk4 protein (arrow).
Application
Mouse Mapk4 Antibody (N-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the Mapk4 antibody detected the Mapk4 protein (arrow).
Application
Mouse Mapk4 Antibody (N-term) (AP14445a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
