Application
Mouse Map4k2 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Map4k2 antibody detected the Map4k2 protein (arrow).| Product Name | Map4k2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Map4k2; Rab8ip; Mitogen-activated protein kinase kinase kinase kinase 2; Germinal center kinase; MAPK/ERK kinase kinase kinase 2; Rab8-interacting protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Rab8ip |
|---|---|
| Alternative Names | Map4k2 Rab8ip Mitogen-activated protein kinase kinase kinase kinase 2 Germinal center kinase MAPK/ERK kinase kinase kinase 2 Rab8-interacting protein |
| Molecular Weight(MW) | 91265 Da |
| Function | Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extend of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN),or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Mediates also the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3 May play a role in the regulation of vesicle targeting or fusion regulation of vesicle targeting or fusion |
| Tissue Specificity | This Mouse Map4k2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 431-459 amino acids from the Central region of human Mouse Map4k2. |
| Cellular Localization | Cytoplasm. Basolateral cell membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein |
| Entrez Gene | 26412 |
|---|---|
| Nucleotide Accession | NP_033032.1 |
Application
Mouse Map4k2 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Map4k2 antibody detected the Map4k2 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
