Application
Mouse Mapk7 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Mapk7 antibody detected the Mapk7 protein (arrow).Application
Mouse Mapk7 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the Mapk7 antibody detected the Mapk7 protein (arrow).Application
Mouse Mapk7 Antibody (N-term) (AP14267a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk7 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Mapk7 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mapk7; Bmk1; Erk5; Mitogen-activated protein kinase 7; Big MAP kinase 1; Extracellular signal-regulated kinase 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Bmk1 Erk5 |
|---|---|
| Alternative Names | Mapk7 Bmk1 Erk5 Mitogen-activated protein kinase 7 Big MAP kinase 1 Extracellular signal-regulated kinase 5 |
| Molecular Weight(MW) | 87733 Da |
| Function | Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras- independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression (By similarity) |
| Tissue Specificity | Detected in testis, brain, kidney, lung and heart. Detected in total embryo (at protein level) |
| Cellular Localization | Cytoplasm. Nucleus. Isoform 2: Nucleus (By similarity). Note=Isoform 2 is detected only in the nucleus. Translocates to the nucleus upon activation (By similarity) |
| Entrez Gene | 23939 |
|---|---|
| Nucleotide Accession | NP_035971.1 |
Application
Mouse Mapk7 Antibody (N-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the Mapk7 antibody detected the Mapk7 protein (arrow).
Application
Mouse Mapk7 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the Mapk7 antibody detected the Mapk7 protein (arrow).
Application
Mouse Mapk7 Antibody (N-term) (AP14267a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk7 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
