Application
Mouse Mapk8 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Mapk8 antibody detected the Mapk8 protein (arrow).| Product Name | Mapk8 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mapk8; Jnk1; Prkm8; Mitogen-activated protein kinase 8; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Jnk1 Prkm8 |
|---|---|
| Alternative Names | Mapk8 Jnk1 Prkm8 Mitogen-activated protein kinase 8 Stress-activated protein kinase JNK1 c-Jun N-terminal kinase 1 |
| Molecular Weight(MW) | 44229 Da |
| Function | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK8/JNK1 In turn, MAPK8/JNK1 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. Phosphorylates the replication licensing factor CDT1, inhibiting the interaction between CDT1 and the histone H4 acetylase HBO1 to replication origins. Loss of this interaction abrogates the acetylation required for replication initiation. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including p53/TP53 and Yes-associates protein YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Contributes to the survival of erythroid cells by phosphorylating the antagonist of cell death BAD upon EPO stimulation. Mediates starvation-induced BCL2 phosphorylation, BCL2 dissociation from BECN1, and thus activation of autophagy Phosphorylates STMN2 and hence regulates microtubule dynamics, controlling neurite elongation in cortical neurons. In the developing brain, through its cytoplasmic activity on STMN2, negatively regulates the rate of exit from multipolar stage and of radial migration from the ventricular zone (By similarity) Phosphorylates several other substrates including heat shock factor protein 4 (HSF4), the deacetylase SIRT1, ELK1, or the E3 ligase ITCH |
| Tissue Specificity | This Mouse Mapk8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 238-268 amino acids from the Central region of Mouse Mapk8. |
| Cellular Localization | Cytoplasm. Nucleus (By similarity). |
| Entrez Gene | 26419 |
|---|---|
| Nucleotide Accession | NP_057909.1 |
Application
Mouse Mapk8 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Mapk8 antibody detected the Mapk8 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
