Application
Mouse Mapk10 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Mapk10 antibody detected the Mapk10 protein (arrow).Application
Mouse Mapk10 Antibody (N-term) (AP14264a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk10 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Mapk10 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mapk10; Jnk3; Prkm10; Serk2; Mitogen-activated protein kinase 10; MAP kinase p49 3F12; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Jnk3 Prkm10 Serk2 |
|---|---|
| Alternative Names | Mapk10 Jnk3 Prkm10 Serk2 Mitogen-activated protein kinase 10 MAP kinase p49 3F12 Stress-activated protein kinase JNK3 c-Jun N-terminal kinase 3 |
| Molecular Weight(MW) | 52532 Da |
| Function | Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress- activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the beta-amyloid precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons |
| Tissue Specificity | Expressed specifically in neurons of the hippocampus, cortex, cerebellum, brainstem, and spinal cord. Seems to be also found in testis, and very weakly in the heart |
| Cellular Localization | Cytoplasm. Membrane; Lipid-anchor. Nucleus. Mitochondrion. Note=Palmitoylation regulates MAPK10 trafficking to cytoskeleton (By similarity). Recruited to the mitochondria in the presence of SARM1 |
Application
Mouse Mapk10 Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Mapk10 antibody detected the Mapk10 protein (arrow).
Application
Mouse Mapk10 Antibody (N-term) (AP14264a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk10 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
