Application
Mouse Mapk9 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Mapk9 antibody detected the Mapk9 protein (arrow).Application
Mouse Mapk9 Antibody (Center) (AP14446c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk9 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Mapk9 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mapk9; Jnk2; Prkm9; Mitogen-activated protein kinase 9; Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Jnk2 Prkm9 |
|---|---|
| Alternative Names | Mapk9 Jnk2 Prkm9 Mitogen-activated protein kinase 9 Stress-activated protein kinase JNK2 c-Jun N-terminal kinase 2 |
| Molecular Weight(MW) | 48189 Da |
| Function | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as proinflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2 In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway Participates also in neurite growth in spiral ganglion neurons |
| Tissue Specificity | All four isoforms are widely distributed in brain. Isoforms alpha-1 and alpha-2 are predominantly expressed in hippocampus, cerebral cortex, caudate-putamen, amygdala and the granule layer of the cerebellum. Alpha-1 is more abundant than alpha-2 in the periaqueductal region and the substantia nigra |
| Cellular Localization | Cytoplasm (By similarity). Nucleus (By similarity) |
| Entrez Gene | 26420 |
|---|---|
| Nucleotide Accession | NP_997575.2 NP_058657.1 |
Application
Mouse Mapk9 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the Mapk9 antibody detected the Mapk9 protein (arrow).
Application
Mouse Mapk9 Antibody (Center) (AP14446c)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk9 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
