Application
Mouse Mapkapk5 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Mapkapk5 antibody detected the Mapkapk5 protein (arrow).Application
Western blot analysis of Mapkapk5 (arrow) using rabbit polyclonal Mouse Mapkapk5 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Mapkapk5 gene.Application
Mouse Mapkapk5 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Mapkapk5 antibody detected the Mapkapk5 protein (arrow).Application
Mouse Mapkapk5 Antibody (N-term) (AP14447a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapkapk5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | Mapkapk5 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mapkapk5; MAP kinase-activated protein kinase 5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | Mapkapk5 MAP kinase-activated protein kinase 5 |
|---|---|
| Molecular Weight(MW) | 54152 Da |
| Function | Tumor suppressor serine/threonine-protein kinase involved in mTORC1 signaling and post-transcriptional regulation Phosphorylates FOXO3, ERK3/MAPK6, ERK4/MAPK4, HSP27/HSPB1, p53/TP53 and RHEB. Acts as a tumor suppressor by mediating Ras- induced senescence and phosphorylating p53/TP53. Involved in post- transcriptional regulation of MYC by mediating phosphorylation of FOXO3: phosphorylation of FOXO3 leads to promote nuclear localization of FOXO3, enabling expression of miR-34b and miR-34c, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent MYC translation. Acts as a negative regulator of mTORC1 signaling by mediating phosphorylation and inhibition of RHEB. Part of the atypical MAPK signaling via its interaction with ERK3/MAPK6 or ERK4/MAPK4: the precise role of the complex formed with ERK3/MAPK6 or ERK4/MAPK4 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPK (ERK3/MAPK6 or ERK4/MAPK4), ERK3/MAPK6 (or ERK4/MAPK4) is phosphorylated and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6 (or ERK4/MAPK4). Mediates phosphorylation of HSP27/HSPB1 in response to PKA/PRKACA stimulation, inducing F-actin rearrangement |
| Tissue Specificity | Expressed ubiquitously. |
| Cellular Localization | Cytoplasm. Nucleus. Note=Translocates to the cytoplasm following phosphorylation and activation. Interaction with ERK3/MAPK6 or ERK4/MAPK4 and phosphorylation at Thr-182, activates the protein kinase activity, followed by translocation to the cytoplasm. Phosphorylation by PKA/PRKACA at Ser-115 also induces nuclear export |
| Entrez Gene | 17165 |
|---|---|
| Nucleotide Accession | XP_001479292.1 NP_034895.1 |
Application
Mouse Mapkapk5 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Mapkapk5 antibody detected the Mapkapk5 protein (arrow).
Application
Western blot analysis of Mapkapk5 (arrow) using rabbit polyclonal Mouse Mapkapk5 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Mapkapk5 gene.
Application
Mouse Mapkapk5 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Mapkapk5 antibody detected the Mapkapk5 protein (arrow).
Application
Mouse Mapkapk5 Antibody (N-term) (AP14447a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapkapk5 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
