Application
Mouse Mastl Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Mastl antibody detected the Mastl protein (arrow).| Product Name | Mastl Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Mastl; Gw; Gwl; Serine/threonine-protein kinase greatwall; Microtubule-associated serine/threonine-protein kinase-like |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Gw Gwl |
|---|---|
| Alternative Names | Mastl Gw Gwl Serine/threonine-protein kinase greatwall Microtubule-associated serine/threonine-protein kinase-like |
| Molecular Weight(MW) | 95975 Da |
| Function | Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited (By similarity) |
| Tissue Specificity | This Mouse Mastl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 560-589 amino acids from the Central region of Mouse Mastl. |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome (By similarity). Nucleus. Note=During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes (By similarity) |
| Entrez Gene | 67121 |
|---|---|
| Nucleotide Accession | NP_080255.3 |
Application
Mouse Mastl Antibody (Center) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the Mastl antibody detected the Mastl protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
